cell cultures human colon cancer cell line sw480  (ATCC)

Journal: Cell reports

Article Title: Suppression of Membranous LRP5 Recycling, Wnt/β-catenin Signaling, and Colon Carcinogenesis by 15-LOX-1 Peroxidation of Linoleic Acid in PI3P

doi: 10.1016/j.celrep.2020.108049

Figure Lengend Snippet: (A and B) LRP5 protein expression in SW480 (A) and LoVo (B) cells stably transduced with control (Ctrl) or 15-LOX-1 lentivirus and treated with 10 μg/mL cycloheximide (CHX) for 0, 2, and 4 h. The whole-cell lysates were analyzed using western blot.

Article Snippet: Cell culture Human colon cancer cell lines SW480 and LoVo and human embryonic kidney cell line 293T were purchased from ATCC and were maintained in McCoy 5A, RPMI 1640, and Dulbecco modified Eagle (high-glucose) media, respectively.

Techniques: Expressing, Stable Transfection, Transduction, Western Blot

Journal: Cell reports

Article Title: Suppression of Membranous LRP5 Recycling, Wnt/β-catenin Signaling, and Colon Carcinogenesis by 15-LOX-1 Peroxidation of Linoleic Acid in PI3P

doi: 10.1016/j.celrep.2020.108049

Figure Lengend Snippet: (A) SNX17, LRP5, and active β-catenin expression in 293T or SW480 cells transfected with control vector (Ctrl) or FLAG-tagged SNX17 expression vector for 48 h, then processed for western blot analysis.

Article Snippet: Cell culture Human colon cancer cell lines SW480 and LoVo and human embryonic kidney cell line 293T were purchased from ATCC and were maintained in McCoy 5A, RPMI 1640, and Dulbecco modified Eagle (high-glucose) media, respectively.

Techniques: Expressing, Transfection, Plasmid Preparation, Western Blot

Journal: Cell reports

Article Title: Suppression of Membranous LRP5 Recycling, Wnt/β-catenin Signaling, and Colon Carcinogenesis by 15-LOX-1 Peroxidation of Linoleic Acid in PI3P

doi: 10.1016/j.celrep.2020.108049

Figure Lengend Snippet: (A and B) SW480 (A) or LoVo (B) cells stably transduced with control (Ctrl) or 15-LOX-1 lentivirus were treated with the PI3P biosynthesis inhibitor VPS34-IN-1 (1 μM) for 2 h and processed for the indicated protein expression using western blot analysis.

Article Snippet: Cell culture Human colon cancer cell lines SW480 and LoVo and human embryonic kidney cell line 293T were purchased from ATCC and were maintained in McCoy 5A, RPMI 1640, and Dulbecco modified Eagle (high-glucose) media, respectively.

Techniques: Stable Transfection, Transduction, Expressing, Western Blot

Journal: Cell reports

Article Title: Suppression of Membranous LRP5 Recycling, Wnt/β-catenin Signaling, and Colon Carcinogenesis by 15-LOX-1 Peroxidation of Linoleic Acid in PI3P

doi: 10.1016/j.celrep.2020.108049

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Cell culture Human colon cancer cell lines SW480 and LoVo and human embryonic kidney cell line 293T were purchased from ATCC and were maintained in McCoy 5A, RPMI 1640, and Dulbecco modified Eagle (high-glucose) media, respectively.

Techniques: Generated, Plasmid Preparation, Recombinant, Modification, Western Blot, Protease Inhibitor, Cell Viability Assay, Magnetic Beads, Expressing, Software

Journal: Cell reports

Article Title: Suppression of Membranous LRP5 Recycling, Wnt/β-catenin Signaling, and Colon Carcinogenesis by 15-LOX-1 Peroxidation of Linoleic Acid in PI3P

doi: 10.1016/j.celrep.2020.108049

Figure Lengend Snippet: (A and B) LRP5 protein expression in SW480 (A) and LoVo (B) cells stably transduced with control (Ctrl) or 15-LOX-1 lentivirus and treated with 10 μg/mL cycloheximide (CHX) for 0, 2, and 4 h. The whole-cell lysates were analyzed using western blot.

Article Snippet: EXPERIMENTAL MODEL AND SUBJECT DETAILS Cell culture Human colon cancer cell lines SW480 and LoVo and human embryonic kidney cell line 293T were purchased from ATCC and were maintained in McCoy 5A, RPMI 1640, and Dulbecco modified Eagle (high-glucose) media, respectively.

Techniques: Expressing, Stable Transfection, Transduction, Western Blot

Journal: Cell reports

Article Title: Suppression of Membranous LRP5 Recycling, Wnt/β-catenin Signaling, and Colon Carcinogenesis by 15-LOX-1 Peroxidation of Linoleic Acid in PI3P

doi: 10.1016/j.celrep.2020.108049

Figure Lengend Snippet: (A) SNX17, LRP5, and active β-catenin expression in 293T or SW480 cells transfected with control vector (Ctrl) or FLAG-tagged SNX17 expression vector for 48 h, then processed for western blot analysis.

Article Snippet: EXPERIMENTAL MODEL AND SUBJECT DETAILS Cell culture Human colon cancer cell lines SW480 and LoVo and human embryonic kidney cell line 293T were purchased from ATCC and were maintained in McCoy 5A, RPMI 1640, and Dulbecco modified Eagle (high-glucose) media, respectively.

Techniques: Expressing, Transfection, Plasmid Preparation, Western Blot

Journal: Cell reports

Article Title: Suppression of Membranous LRP5 Recycling, Wnt/β-catenin Signaling, and Colon Carcinogenesis by 15-LOX-1 Peroxidation of Linoleic Acid in PI3P

doi: 10.1016/j.celrep.2020.108049

Figure Lengend Snippet: (A and B) SW480 (A) or LoVo (B) cells stably transduced with control (Ctrl) or 15-LOX-1 lentivirus were treated with the PI3P biosynthesis inhibitor VPS34-IN-1 (1 μM) for 2 h and processed for the indicated protein expression using western blot analysis.

Article Snippet: EXPERIMENTAL MODEL AND SUBJECT DETAILS Cell culture Human colon cancer cell lines SW480 and LoVo and human embryonic kidney cell line 293T were purchased from ATCC and were maintained in McCoy 5A, RPMI 1640, and Dulbecco modified Eagle (high-glucose) media, respectively.

Techniques: Stable Transfection, Transduction, Expressing, Western Blot

Journal: Cell reports

Article Title: Suppression of Membranous LRP5 Recycling, Wnt/β-catenin Signaling, and Colon Carcinogenesis by 15-LOX-1 Peroxidation of Linoleic Acid in PI3P

doi: 10.1016/j.celrep.2020.108049

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: EXPERIMENTAL MODEL AND SUBJECT DETAILS Cell culture Human colon cancer cell lines SW480 and LoVo and human embryonic kidney cell line 293T were purchased from ATCC and were maintained in McCoy 5A, RPMI 1640, and Dulbecco modified Eagle (high-glucose) media, respectively.

Techniques: Generated, Plasmid Preparation, Recombinant, Modification, Western Blot, Protease Inhibitor, Cell Viability Assay, Magnetic Beads, Expressing, Software